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Tumour specific immunogenicity of methylcholanthrene-induced sarcoma cells after incubation in neuraminidase.

British Journal of Cancer
Nature Publishing Group
Publication Date
  • Research Article
  • Biology
  • Chemistry
  • Medicine


141 TUMOUR SPECIFIC IMMUNOGENICITY OF METHYLCHOLANTHRENE-INDUCED SARCOMA CELLS AFTER INCUBATION IN NEURAMINIDASE G. A. CURRIE AND K. D. BAGSHAWE From the Edgar and Tenovus Laboratories, Charing Cross Group of Hospitals, Fulham Hospital, London, W.6. Received for publication October 31, 1968 IT was previously suggested (Currie and Bagshawe, 1967) that effective anti- genic expression by some types of tumour cell may be inhibited by the presence of sialic (N-acetylneuraminic) acid in the cell periphery. Treatment of the cells from several " non-specific " transplantable mouse tumours with neuraminidase results in a marked increase in their immunogenicity when subsequently injected into intact host mice and leads to a powerful anti-tumour immunity in these animals. So far this phenomenon has been reported using the Ehrlich (Linden- mann and- Klein, 1967), Landschuitz (Currie, 1967), and TA3 (Sanford, 1967) ascitic tumours. The antigenic discrepancies between these tumours and their respective host mice are probably related to strain-specific histocompatibility differences, i.e. these tumours are malignant allografts. Little of the information obtained from the study of such tumours is of immediate relevance to the growth and development of autochthonous tumours where the only tumour-host antigen discrepancy (if any) is specific to the tumour. The purpose of this paper is to report studies of the effects of incubation in neuraminidase on the immunogenicity of chemically-induced tumour cells trans- planted to mice of identical genotype; a situation in which a possible role for sialic acid in the effective expression oftumour specific antigens can be investigated. MATERIALS AND METHODS Mice.-All the animals used in this study were young adult male inbred CBA strain mice. This mouse colony has been maintained by sib-sib mating in these laboratories for five years and random skin-grafting has indicated that the colony represents an immunologically homogeneous population. For use in these exp

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