Affordable Access

Publisher Website

A closed flow chamber for long-term multichannel recording and optical monitoring

Authors
Journal
Journal of Neuroscience Methods
0165-0270
Publisher
Elsevier
Publication Date
Volume
52
Issue
1
Identifiers
DOI: 10.1016/0165-0270(94)90059-0
Keywords
  • Neuronal Network
  • Neuronal Culture
  • Long-Term Recording
  • Culture Life-Support
Disciplines
  • Biology
  • Design
  • Medicine
  • Pharmacology

Abstract

Abstract An autoclavable chamber and associated medium circulation system has been constructed to provide a stable environment for mammalian cultures for long periods of time. The chamber was specifically designed for (a) multichannel electrophysiological recording from monolayer networks with photoetched multielectrode matrices, (b) for microscope observations of networks in the living state and (c) for the manipulation of such networks with laser cell surgery. The chamber components can be assembled under sterile conditions in less than 30 s to minimize pH and osmolarity stress to the monolayer cultures. An open chamber version provides a constant medium bath for pharmacological studies. The closed chamber version, attached to a 10-ml medium reservoir and a peristaltic pump, has so far provided constant conditions for continual recording over an 8-day period. Flow characteristics within the closed chamber, optical properties, pH maintenance, and schemes for drug addition are described.

There are no comments yet on this publication. Be the first to share your thoughts.