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Mutational analysis of an archaeal minichromosome maintenance protein exterior hairpin reveals critical residues for helicase activity and DNA binding.

Authors
  • 1
Type
Published Article
Journal
BMC Molecular Biology
1471-2199
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
11
Pages
62–62
Identifiers
DOI: 10.1186/1471-2199-11-62
PMID: 20716382
Source
Medline

Abstract

These results implicate several current theories regarding helicase activity by this critical hexameric enzyme. As the data suggest that EXT-hp is involved in DNA binding, the results reported here imply that the EXT-hp located near the exterior exit of the side channels may play a role in contacting DNA substrate in a manner that affects DNA unwinding.

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