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Three-dimensional architecture of rat glomerular basement membrane by ultra-high resolution scanning electron microscopy.

Authors
Publisher
Okayama University Medical School
Publication Date
Keywords
  • Glomerular Basement Membrance
  • Ultrastructure
  • Ultra-High Resolution Scanning Electron Microscopy
  • Conductive Staining

Abstract

We demonstrated the ultrastructure of rat glomerular basement membrane (GBM) by ultra-high resolution scanning electron microscopy. GBM prepared by sonication methods and conductive-staining could be observed without metal coating at magnifications as high as 400,000 times. The GBM showed an irregular meshwork structure composed of various strands and pores. The width of the strands ranged from 6 to 15 nm, and the diameter of pores ranged from 6 to 50 nm. The present study confirmed our molecular sieve theory of the basement membrane.

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