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The chloroplast 32 kDa protein is synthesized on thylakoid-bound ribosomes inChlamydomonas reinhardtii

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
184
Issue
1
Identifiers
DOI: 10.1016/0014-5793(85)80660-8
Keywords
  • Chlamydomonas
  • Psba Gene
  • Dn A-Rna Hybridization
  • Rough Thylakoid
  • In Vitro Protein Synthesis
  • 32 Kda Precursor
Disciplines
  • Biology

Abstract

Abstract A cloned cpDNA fragment containing a portion of the gene for the 32–36 kDa thylakoid protein of Chlamydomonas (polypeptide D-1) was isolated. Hybridization probing of RNA from soluble and membrane fractions of Chlamydomonas showed that the mRNA for D-1 is bound to thylakoid membranes. Run-off translation of thylakoid-bound polysomes (rough thylakoids) with [ 35S]-methionine yields polypeptide D-1 as the major product. Peptide mapping with S. aureus V-8 protease of D-1 synthesized (1) in vivo, (2) in vitro by rough thylakoids and (3) in the reticulocyte lysate directed by non-polyadenylated RNA showed that D-1 is synthesized as a precursor in the reticulocyte lysate but as the mature polypeptide by rough thylakoids.

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