Affordable Access

Publisher Website

Multichannel microspectrofluorometry for topographic and spectral analysis of NAD(P)H fluorescence in single living cells

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Bioenergetics
0005-2728
Publisher
Elsevier
Publication Date
Volume
396
Issue
1
Identifiers
DOI: 10.1016/0005-2728(75)90198-x

Abstract

Abstract Starting from a previously described prototype microspectrofluorometer a more versatile apparatus has been developed with rapid optional operation on a topographic mode for the simultaneous multisite evaluation of NAD(P) reduction-reoxidation transients or on a spectral mode for the analysis of natural and exogenous fluorochromes, in single living cells. On the topographic mode, a detailed kinetic analysis of NAD or NAD P-linked dehydrogenases can be made from 50–100 cell points simultaneously via automatic recording of topographic scans up to 16 times a second, in correlation with microelectrophoretic intracellular injection of metabolites (e.g. nearly immediate response to glucose 6-phosphate, 20–25 s delay for 6-phosphogluconate). Rapid shifts from topographic to spectral operation make possible the detection of a change in fluorescence intensity at a specific intracellular site and the immediate verification of its nature (NAD(P)H or exogenous fluorochrome) by spectral observation,

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Multichannel microspectrofluorometry for topograph...

on Biochimica et Biophysica Acta Jul 08, 1975

32-Msec scan of the NAD(P)H fluorescence spectrum...

on Review of Scientific Instrumen... December 1973

Bimolecular fluorescence complementation analysis...

on Drug metabolism and dispositio... September 2005

Rapid multichannel microfluorometry for metabolic...

on Experimental Cell Research Jan 01, 1973
More articles like this..