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Channel catfish virus: Physicochemical properties of the viral genome and identification of viral polypeptides

Authors
Journal
Virology
0042-6822
Publisher
Elsevier
Publication Date
Volume
103
Issue
2
Identifiers
DOI: 10.1016/0042-6822(80)90186-5
Disciplines
  • Biology
  • Chemistry
  • Design

Abstract

Abstract This investigation describes the kinetics of synthesis and physicochemical properties of the channel catfish virus (CCV) genome as well as the nature of virus-specific polypeptides. Sucrose density gradient sedimentation and contour length measurements of CCV DNA revealed that the genome possesses a molecular weight of 85 ± 4 × 10 6, while reassociation kinetics demonstrated the kinetic complexity to be 78 ± 5 × 10 6. Another feature of the CCV genome revealed by these studies is that it is linear and nonpermuted: restriction enzyme analysis indicated that all fragments were present in unimolar amounts, the summed molecular weights of which equaled that of the intact genome. Thirty-two polypeptides appearing in infected cells were designated virus-specific. These polypeptides possess molecular weights ranging from 12,000 to 300,000. Eighteen of the polypeptides were identified as structural components of purified virions. Kinetic analysis of the synthesis of viral polypeptides revealed three distinct groups of proteins differing in the time of their appearance and magnitude of synthesis. The kinetics of synthesis of CCV-specific proteins suggests that their synthesis is coordinately regulated.

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