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The determination of glycopeptides by liquid chromatography/mass spectrometry with collision-induced dissociation

Authors
Journal
Journal of the American Society for Mass Spectrometry
1044-0305
Publisher
Springer-Verlag
Publication Date
Volume
3
Issue
8
Identifiers
DOI: 10.1016/1044-0305(92)80003-4
Disciplines
  • Biology

Abstract

Abstract Glycopeptides derived from ribonuclease B and ovomucoid have been subjected to collision-induced dissociation (CID) in the second quadrupole of a triple quadrupole mass spectrometer. Doubly charged parent ions gave predictable fragmentation that yielded partial sequence information of the attached oligosaccharide as Hex and HexNAc units. Common oxonium ions are observed in the product ion mass spectra of the glycopeptides that correspond to HexNAc + ( m/z 204) and HexHexNAc + ( m/z 366). A strategy for locating the glycopeptides in the proteolytic digest mixtures of glycoproteins by ions spray liquid chromatography mass spectrometry (LC/MS) is described by utilizing CID in the declustering region of the atmospheric pressure ionization mass spectrometer to produce these characteristic oxonium ions. This LC/CID/MS approach is used to identify glycopeptides in proteolytic digest mixtures of ovomucoid, asialofetuin, and fetuin. LC/CID/MS in the selected ion monitoring mode may be used to identify putative glycopeptides from the proteolytic digest of fetuin.

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