Affordable Access

Publisher Website

Determination of the agonist-antagonist anxiolytic agent 6-(2-chlorophenyl)-4-hydroxy-4H-imidazo[1,5-a]-[1,4]benzodiazepine-3-carboxamide and its benzophenone carboxylic acid metabolite in plasma by high-performance liquid chromatography

Authors
Journal
Journal of Chromatography B Biomedical Sciences and Applications
0378-4347
Publisher
Elsevier
Publication Date
Volume
417
Identifiers
DOI: 10.1016/0378-4347(87)80128-7
Disciplines
  • Physics

Abstract

Abstract A rapid, sensitive and selective high-performance liquid chromatographic (HPLC) assay was developed for the determination of 6-(2-chlorophenyl)-4-hydroxy-4 H-imidazo[1,5- a]-[1,4]benzodiazepine-3-carboxamide (I) and its benzophenone carboxylic acid metabolite, 1-[2-(2-chlorobenzoyl)phenyl]-4-(aminocarbonyl)-1 H-imidazole-5-carboxylic acid (II) in plasma. The assay involves the extraction of both compounds into benzene from buffered plasma (pH 5.4) and subsequent analysis by reversed-phase HPLC. The overall recovery of I and II is 98.3 ± 9.4 and 59.7 ± 15.7% for dog plasma, 86.0 ± 14.7 and 52.8 ± 15.1% for rat plasma and 98.1 ± 9.3 and 66.9 ± 18.0% for human plasma, respectively. The sensitivity limit of the assay for I and II is 20.0 and 40.0 ng/ml of plasma using ultraviolet detection at 254 nm. The assay was used in studies in dog and rat.

There are no comments yet on this publication. Be the first to share your thoughts.