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Tumour promoter induces proliferation of 3T6 cells in serum-free medium, as do polypeptide growth factors

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
130
Issue
2
Identifiers
DOI: 10.1016/0014-4827(80)90030-0

Abstract

Abstract The potent tumour promoter agent 12- O-tetradecanoyl-phorbol-13-acetate (TPA) induces multiple rounds of 3T6 cell replication in completely serum-free medium. The maximal effect is elicited at 100 ng/ml, is further enhanced by adding insulin, and is comparable to that of the polypeptide's epidermal growth factor (EGF), insulin, and fibroblast-derived growth factor. The results support the proposition that TPA acts as a mitogen via mechanisms akin to those used by polypeptide growth factors.

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