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Molecular Cloning of a cDNA Encoding the AmphibianPleurodeles waltl70-kDa Heat-Shock Cognate Protein

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
238
Issue
1
Identifiers
DOI: 10.1006/bbrc.1997.7255
Disciplines
  • Biology

Abstract

Abstract We isolated and characterized a cDNA coding for heat-shock protein 70 of the amphibianPleurodeles waltl.This 2212-bp sequence exhibited one open reading frame of 645 amino acids. The predicted amino acid sequence exhibited the three conserved elements of the HSC/HSP70 protein family. Comparison of nucleotide and amino acid sequences between this gene and otherhsc/hsp-likegenes revealed a high identity with the cognate form HSC70. Byin vitrotranslation, this gene encoded a 70-kDa protein which was different than the induciblePleurodeles waltlHSP70 protein. This translated protein was recognized byPleurodeles waltlN1 anti-HSC/HSP70 antibody. Heat-inducibility tests showed that this gene was constitutively expressed during oogenesis and embryogenesis, and its expression was not increased after a heat-shock. These results led us to conclude that we recovered aPleurodeles waltlcognatehsc70gene.

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