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Differential up-regulation of voltage-dependent na+channels induced by phenytoin in brains of genetically seizure-susceptible (el) and control (ddy) mic

Elsevier Ltd
Publication Date
DOI: 10.1016/0306-4522(94)90478-2


Abstract We investigated the effect of in vivo administration of an antiepileptic drug, phenytoin, on the saxitoxin binding capacity of receptor site 1 of the Na + channel α-subunit, and the expression activity of the channel messenger RNA in epileptic El mouse brains, as compared with parental ddY mice. Subchronic treatment with phenytoin (25 mg/kg per day) for 14 days increased the [ 3H]saxitoxin binding to brain-derived synaptic membranes of both El and control ddY mice in a time dependent manner. This increase plateaued at 21 ± 4% in El mice and 28 ± 3% in ddY control mice after administration of phenytoin for seven days. After cessation of treatment with phenytoin, [ 3H]saxitoxin binding capacity returned to the basal level within two weeks in both ddY and El brains. Scatchard plot analysis revealed that the phenytoin treatment caused a 20–30% increase in maximum binding capacity of [ 3H]saxitoxin binding without any change in equilibrium dissociation constant in the brain cortical synaptic membranes of both epileptic El and control ddY mice. A single injection of phenytoin (25 mg/kg) elevated the level of Na + channel messenger RNA within l h in ddY mouse brains. The increase in Na + channel messenger RNA reached a peak (about 80% increase) after 5 h of phenytoin administration in a concentration-dependent manner (6.25–50 mg/kg). On the other hand, in El mouse brains, Na + channel messenger RNA was not elevated until more than 5 h after phenytoin injection, and was increased by only about 33%. These results indicate that subchronic administration of phenytoin enhanced the expression of voltage-dependent Na + channel messenger RNA, which was followed by an increase in the number of Na + channels in both El and ddY mice. However, a single injection of phenytoin revealed a delayed expression of Na + channel messenger RNA in El mouse brain, compared to ddY brains, suggesting that the molecular basis for up-regulation of Na + channels in epileptic El brains is abnormal.

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