Affordable Access

Publisher Website

Evaluation of ferritin-overexpressing brain in newly developed transgenic mice

Authors
Journal
Magnetic Resonance Imaging
0730-725X
Publisher
Elsevier
Publication Date
Volume
29
Issue
2
Identifiers
DOI: 10.1016/j.mri.2010.08.013
Keywords
  • 1H-Mrs
  • Ferritin
  • Iron
  • Transgenic Mouse
Disciplines
  • Biology
  • Medicine

Abstract

Abstract Aberrant expression of ferritin, a major iron-binding protein, has shown to be involved in neurodegenerative diseases. In this study, we generated transgenic (Tg) mice of human ferritin heavy chain (FTH) gene and investigated the effects of ferritin overexpression in FTH-Tg brain by 1H-MRI and 1H-MRS. The mice displayed no apparent neurological symptoms, and no specific morphological and T 2 alterations were found in the brain by MRI, and not even by histological studies. 1H-MRS, however, revealed that some metabolic markers were significantly altered in FTH-Tg brains compared to wild-type control brains, such as decreases in myo-inositol and glutamine, and an increase in lactate. Our present studies suggested that despite the absence of neurological, morphological, T 2, and histological signatures, brain metabolisms were significantly affected in FTH-Tg mice. This study also highlights the usefulness of 1H-MRS in the analysis of transgenic mouse models.

There are no comments yet on this publication. Be the first to share your thoughts.