Affordable Access

Publisher Website

Vasoconstrictor-induced protein-tyrosine phosphorylation in cultured vascular smooth muscle cells

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
285
Issue
1
Identifiers
DOI: 10.1016/0014-5793(91)80721-e
Keywords
  • Vasoconstrictor
  • Tyrosine Phosphorylation
  • Protein Kinase C
  • Calcium
  • Vascular Smooth Muscle Cell
Disciplines
  • Biology

Abstract

Abstract In cultured rat aortic smooth muscle cells, angiotensin II induced tyrosine phosphorylation of at least 9 proteins with molecular masses of 190, 117, 105, 82, 79, 77, 73, 45 and 40 kDa in time- and dose-dependent manners. Other vasoconstrictors such as [Arg]vasopressin, 5-hydroxytryptamine and norepinephrine induced the tyrosine phosphorylation of the same set of proteins as angiotensin II. The tyrosine phosphorylation of these proteins was mimicked by the protein kinase C-activating phorbol ester, phorbol 12 myristate, 13-acetate, and the Ca 2+ ionophore, ionomycin. These results demonstrate that the vasoconstrictors stimulate the tyrosine phosphorylation of several proteins in vascular smooth muscle cells and suggest that the tyrosine phosphorylation reactions are the events distal to the activation of protein kinase C and Ca 2+ mobilization in the intracellular signalling pathways of the vasoconstrictors.

There are no comments yet on this publication. Be the first to share your thoughts.