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Total particulate carbon and nitrogen at station DI183_11869#25

Publication Date
DOI: 10.1594/pangaea.206273
  • 11869#25
  • Biogeochemical Ocean Flux Study
  • Bofs
  • Carbon
  • Total Particulate
  • D183
  • Di183_11869#25
  • Discovery (1962)
  • Jgofs
  • Joint Global Ocean Flux Study
  • Nitrogen
  • Total Particulate
  • Stand-Alone Pumps


Pigments Introduction This document covers the pigment data held in files PIGMENT1, PIGMENT2 and PIGMENT3. Water samples were obtained for pigment measurements using Challenger Oceanics stand-alone pumps (SAPs). These are self contained submersible pumps which were lowered to the required depth on a kevlar wire. Large volumes of water were then pumped through the integral filters. In the case of pigment samples, GF/F filters were used. Pigment Determinations by HPLC The frozen filters were extracted in 2-5ml 90% acetone using sonification, and centrifuged to remove cellular debris. A 300 µl aliquot of clarified extract was mixed with 300 µl of 1M ammonium acetate and 100 µl injected into a Shimadzu HPLC system (dual LC-6A pumps, SPD-6AV spectrophotometric detector, SCL-6B system controller) incorporating a 3-µm Pecosphere column (3.5x0.45 cm, Perkin- Elmer). Pigments were separated by a modification of the reversed-phase method of Mantoura and Llewellyn (1983): solvent A consisted of 80% methanol and 20% 1M ammonium acetate and solvent B contained 60% methanol and 40% acetone. A linear gradient from 0% B to 100% B for 10 min followed by an isocratic hold at 100% B for 7.5 minutes was used at a flow rate of 1 ml per minute. Chlorophylls and carotenoids were detected by absorbance at 440 nm, and a detection of phaeopigments was performed with a Perkin-Elmer LS1 fluorescence detector using an excitation wavelength of 400 (±20) nm and emission at >600nm. Dual channel data collection and integration utilised the Philips PU6000 software running on a Dell PC. Pigments were identified by comparison of retention times of various pigments isolated from well documented microalgal species in the Plymouth Culture Collection. Peak identity was further confirmed on selected samples by on-line diode array spectroscopy using a Waters 990 diode array spectrophotometric detector. Quantification of pigments was based on peak areas and extinction coefficients updated from those reported by Mantoura

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