Affordable Access

Publisher Website

Large-scale preparation of recombinant human calcitonin from a multimeric fusion protein produced inEscherichia coli

Authors
Publisher
Elsevier B.V.
Publication Date
Volume
87
Issue
3
Identifiers
DOI: 10.1016/s1389-1723(99)80035-8
Keywords
  • Human Calcitonin
  • Multimeric Fusion Protein
  • Citraconilation
Disciplines
  • Biology

Abstract

Abstract In order to develop a large-scale, high-yield production process for human calcitonin (hCT) in Escherichia coli, a stable expression plasmid was constructed and the expressed protein was modified for efficient cleavage by protease. Multiple copies of a synthetic gene encoding hCT-Leu-Arg, a substrate for C-terminal amidation by carboxypeptidase Y (CPY), were inserted into the stable expression plasmid. Using this plasmid, the expression of a multimeric fusion protein was induced by shifting the temperature from 34 to 38°C. The multimeric fusion protein was accumulated as inclusion bodies. After the fermentation, the cells were harvested and disrupted by a homogenizer. The insoluble multimeric fusion protein was suspended in 6.6 M urea solution. At this time, however, the protein could not be solubilized and thus the efficiency of cleavage by protease was low. To solubilize the protein and protect Lys residues against digestion by trypsin, the protein was citraconilated with citraconic anhydride. Following S-sulfonation with Na 2SO 3-CuSO 4, almost all the protein was solubilized. The solubilized, citraconilated, and S-sulfonated protein was digested with trypsin efficiently. By treatment with trypsin, the multimeric fusion protein was cleaved into monomers of citraconilated and S-sulfonated hCT-Leu-Arg (S-hCT-Leu-Arg). The subsequent decitraconilation was performed at low pH. S-hCT-Leu-Arg, isolated by preparative HPLC, was directly converted into S-hCT-HN 2 by CPY without removal of the Arg residue. Finally, S-hCT-NH 2 was desulfonated and converted into mature hCT. In this way, a large amount of recombinant mature hCT was obtained in a tank fermentation. To our knowledge, this is the first report of industrial-scale, human calcitonin production using a multimeric fusion protein expression system.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Large-scale preparation of recombinant human calci...

on Journal of Bioscience and Bioe... 1999

Production of human calcitonin inEscherichia colif...

on Journal of Fermentation and Bi... Jan 01, 1996

Large scale purification of recombinant insulin-li...

on Journal of Fermentation and Bi... Jan 01, 1996

High-level synthesis inEscherichia coliof recombin...

on Journal of Bioscience and Bioe... Jan 01, 1991
More articles like this..