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Solution structure of dAATAA and dAAUAA DNA bulges

Nucleic Acids Research
Oxford University Press
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  • Article
  • Biology
  • Chemistry


OP-NARE140011 4294..4305 A bacteriophage transcription regulator inhibits bacterial transcription initiation by p-factor displacement Bing Liu1,y, Andrey Shadrin1,y, Carol Sheppard1, Vladimir Mekler2, Yingqi Xu1, Konstantin Severinov2,3, Steve Matthews1,* and Sivaramesh Wigneshweraraj1,* 1MRC Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK, 2Waksman Institute for Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, NJ USA and 3St. Petersburg State Polytechnical University, St. Petersburg, Russia Received November 8, 2013; Revised December 4, 2013; Accepted December 30, 2013 ABSTRACT Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic b and b’ subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the p70-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription. INTRODUCTION Bacteriophages (phages) use an impressive array of mechanisms to inactivate or repurpose bacterial processes for their own developmental needs (1,2). Many phage genomes encode small proteins that specifically affect the multisubunit RNA polymerase (RNAp) of the bacterial host, inhibiting bacterial DNA transcription while promoting regulated phage DNA transcription (1,2). Illuminatin

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