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Presence of β-Lactamase Gene TEM-1 DNA Sequence in Commercial Taq DNA Polymerase

Authors
Publisher
American Society for Microbiology
Publication Date
Source
PMC
Keywords
  • Letters To The Editor

Abstract

PCR products amplified from negative (water) controls with primers targeting the blaTEM gene. Chiang C et al. J. Clin. Microbiol. 2005;43:530-531 PCR products amplified from negative (water) controls with primers targeting the blaTEM gene. The product was run on a 1% agarose gel with 0.5× Tris-acetate-EDTA buffer. All reactions were performed in duplicate. Lane M, DNA size marker; lanes 1 and 2, PCR buffer from manufacturer B with Taq DNA polymerase from manufacturer B; lanes 3 and 4, PCR buffer from manufacturer B with Taq DNA polymerase from manufacturer A; lanes 5 and 6, PCR buffer from manufacturer A with Taq DNA polymerase from manufacturer B; lanes 7 and 8, PCR buffer from manufacturer A with Taq DNA polymerase from manufacturer A. The arrow indicates the amplified blaTEM-1 gene fragment.

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