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The binding of a ligand to an acceptor undergoing indefinite self-association

Authors
Journal
Journal of Theoretical Biology
0022-5193
Publisher
Elsevier
Publication Date
Volume
117
Issue
4
Identifiers
DOI: 10.1016/s0022-5193(85)80241-1

Abstract

Explicit expressions are derived which describe the binding of a univalent ligand to equivalent and independent sites on each state of an acceptor undergoing indefinite self-association that is governed by an isodesmic equilibrium constant K I . From considerations of systems in which the same site-binding constant k A applies to all acceptor-ligand interactions, the general forms of binding curves and Scatchard plots are deduced for situations in which binding sites are either created or lost at each monomer-monomer interface. Greater generality is then introduced into the model by allowing ligand interactions with polymeric acceptor states to be governed by a site-binding constant k P that differs in magnitude from that for monomeric acceptor, k A . Finally, experimental results with the glutamate dehydrogenase-GTP and lysozyme-saccharide systems are used to illustrate ways in which the present quantitative expressions may be applied to the characterization of interactions between a ligand and an indefinitely self-associating acceptor.

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