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Proteome analysis of cerebrospinal fluid in Guillain–Barré syndrome (GBS)

Authors
Journal
Journal of Neuroimmunology
0165-5728
Publisher
Elsevier
Publication Date
Volume
185
Identifiers
DOI: 10.1016/j.jneuroim.2007.01.022
Keywords
  • Guillain–Barré Syndrome
  • Proteomics
  • 2-D-Difference In-Gel Electrophoresis
  • Cerebrospinal Fluid
Disciplines
  • Biology
  • Medicine

Abstract

Abstract We used two-dimensional difference in-gel electrophoresis (2-D-DIGE) for proteome analysis of cerebrospinal fluid (CSF) in Guillain–Barré syndrome (GBS). Spots showing > 2-fold difference between GBS and controls were analysed using MALDI-TOF mass spectrometry. Proteins that were up-regulated in GBS included haptoglobin, serine/threonine kinase 10, α-1-antitrypsin, SNC73, alpha II spectrin, IgG kappa chain and cathepsin D preprotein, while transferrin, caldesmon, GALT, human heat shock protein 70, amyloidosis patient HL-heart-peptide 127aa and transthyretin were down-regulated. Some of these proteins are reported in CSF of GBS for the first time. Accordingly, the 2-D-DIGE technology may be useful to identify disease-specific proteins in patients with GBS.

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