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Conformational studies of d(CACGTG)2 and d(CACCGTG).d(CACGGTG) by two-dimensional 1h, 31p NMR and circular dichroism spectra

Authors
Journal
Tetrahedron
0040-4020
Publisher
Elsevier
Publication Date
Volume
46
Issue
4
Identifiers
DOI: 10.1016/s0040-4020(01)86695-3

Abstract

Abstract The conformational studies of synthetic oligonucleotides d(CACGTG)2 and d(CACCGTG).d(CACGGTG) by two-dimensional 1H NMR (COSY and NOESY), 31P NMR and CD spectroscopic methods are reported. The results suggest that these DMA duplexes adopt B-form globally and significant microheterogeneity persists in local conformation. In particular, the sugars of nucleotides C3 of the hexamer and C3, C10 of the heptamer exhibit 3'-endo conformations. These sequences fail to undergo B-Z transition in solution though, d(CACGTG)2 is reported to crystallise in a left handed form.

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