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Autoradiographic localization of benzodiazepine receptor binding in dissociated cultures of fetal mouse cerebral cortex.

Authors
  • Sher, P K
  • Neale, E A
  • Machen, V L
Type
Published Article
Journal
Journal of neurochemistry
Publication Date
Mar 01, 1986
Volume
46
Issue
3
Pages
899–904
Identifiers
PMID: 3005496
Source
Medline
License
Unknown

Abstract

Autoradiography utilizing photoaffinity labelling with [3H]flunitrazepam was used in living cultures of fetal mouse cerebral cortex in situ to localize benzodiazepine receptor binding sites. There was a predominant localization of silver grains over neurons; however, substantial labelling also occurred over nonneuronal background cells. Clonazepam (0.1 microM) and Ro 5-4864 (0.1 microM) displaced substantial numbers of silver grains over neurons and background cells, respectively. In addition, clonazepam displaced 58-68% of specific grains over background cells and Ro 5-4864 displaced 30% of grains over neurons, suggesting that multiple cell types in the CNS may participate in the neuropharmacologic actions of the benzodiazepines.

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