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Autonomous and 3D real-time multi-beam manipulation in a microfluidic environment

Authors
  • Perch-Nielsen, I.
  • Rodrigo, P.J.
  • Alonzo, C.A.
  • Glückstad, J.
Publication Date
Dec 11, 2006
Source
Online Research Database In Technology
Keywords
Language
English
License
Unknown
External links

Abstract

The Generalized Phase Contrast (GPC) method of optical 3D manipulation has previously been used for controlled spatial manipulation of live biological specimen in real-time. These biological experiments were carried out over a time-span of several hours while an operator intermittently optimized the optical system. Here we present GPC-based optical micromanipulation in a microfluidic system where trapping experiments are computer-automated and thereby capable of running with only limited supervision. The system is able to dynamically detect living yeast cells using a computer-interfaced CCD camera, and respond to this by instantly creating traps at positions of the spotted cells streaming at flow velocities that would be difficult for a human operator to handle. With the added ability to control flow rates, experiments were also carried out to confirm the theoretically predicted axially dependent lateral stiffness of GPC-based optical traps. (c) 2006 Optical Society of America

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