Automatic Calculation of the Kinetic Parameters of Enzymatic Reactions with Their Standard Errors Using Microsoft Excel
- Authors
- Type
- Published Article
- Journal
- Journal of Applied Glycoscience
- Publisher
- The Japanese Society of Applied Glycoscience
- Publication Date
- Mar 03, 2023
- Volume
- 70
- Issue
- 1
- Pages
- 33–37
- Identifiers
- DOI: 10.5458/jag.jag.JAG-2022_0012
- PMID: 37033113
- PMCID: PMC10074026
- Source
- PubMed Central
- Keywords
- Disciplines
- License
- Unknown
Abstract
We created a Microsoft Excel file, Enzyme_Kinetics_Calculator, which includes macro programs that automatically calculates kinetic parameters for typical kinetic equations of enzymatic reactions, accompanied by their standard errors, by minimizing the residual sum of squares thereof. The [ S ]- v plot is automatically drawn with the theoretical lines and, similarly, the 1/[ S ]-1/ v plot in the case of linear theoretical lines. Enzyme_Kinetics_Calculator is available as a supplementary file for this paper (see J. Appl. Glycosci. Web site).
