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An automated Teflon microfluidic peptide synthesizer.

Authors
  • Zheng, Hui
  • Wang, Weizhi
  • Li, Xiaojun
  • Wang, Zihua
  • Hood, Leroy
  • Lausted, Christopher
  • Hu, Zhiyuan
Type
Published Article
Journal
Lab on a Chip
Publisher
The Royal Society of Chemistry
Publication Date
Sep 07, 2013
Volume
13
Issue
17
Pages
3347–3350
Identifiers
DOI: 10.1039/c3lc50632k
PMID: 23835869
Source
Medline
License
Unknown

Abstract

We present a microfluidic synthesizer made entirely of Teflon material for solid phase peptide synthesis (SPPS). Solvent-resistant perfluoroalkoxy (PFA) was used to construct chip-sized devices featuring multiple tri-layer pneumatic microvalves. Using these devices, model peptides were automatically synthesized and cleaved in situ in a continuous-flow manner. The total coupling and cleavage time was significantly reduced compared to conventional bulk reactors. The synthesis of a decapeptide, for instance, took less than 6 h using our device while it usually takes more than three days using conventional reactors.

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