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Automated sampling and data processing derived from biomimetic membranes

  • Perry, Mark
  • Vissing, Thomas
  • Boesen, P.
  • Hansen, Jesper Søndergaard
  • Emnéus, Jenny
  • Helix Nielsen, Claus
Publication Date
Jan 01, 2009
Online Research Database In Technology
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Recent advances in biomimetic membrane systems have resulted in an increase in membrane lifetimes from hours to days and months. Long-lived membrane systems demand the development of both new automated monitoring equipment capable of measuring electrophysiological membrane characteristics and new data processing software to analyze and organize the large amounts of data generated. In this work, we developed an automated instrumental voltage clamp solution based on a custom-designed software controller application (the WaveManager), which enables automated on-line voltage clamp data acquisition applicable to long-time series experiments. We designed another software program for off-line data processing. The automation of the on-line voltage clamp data acquisition and off-line processing was furthermore integrated with a searchable database (DiscoverySheet (TM)) for efficient data management. The combined solution provides a cost efficient and fast way to acquire, process and administrate large amounts of voltage clamp data that may be too laborious and time consuming to handle manually.

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