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Automated analysis of mitomycin C in body fluids by high-performance liquid chromatography with on-line sample pre-treatment.

Authors
  • Tjaden, U R
  • de Bruijn, E A
  • van der Hoeven, R A
  • Jol, C
  • van der Greef, J
  • Lingeman, H
Type
Published Article
Journal
Journal of chromatography
Publication Date
Sep 04, 1987
Volume
420
Issue
1
Pages
53–62
Identifiers
PMID: 3117825
Source
Medline
License
Unknown

Abstract

A fully automated liquid chromatographic system for the bioanalysis of mitomycin C has been described. The isolation of the analyte from the biological matrix (plasma, ascites and urine) is performed using a continuous-flow system equipped with a dialysis membrane in order to remove proteins. The samples are concentrated on a reversed-phase pre-column and subsequently introduced on to a reversed-phase analytical column by applying column-switching techniques. The drug is detected by absorbance measurements at 360 nm. Using the described system up to 100 samples a day can be analysed with determination limits of the order of 1 ng/ml, with a linear dynamic range of at least three decades for plasma and urine samples. The procedure was applied to pharmacokinetic studies of ovarian cancer patients treated intraperitoneally with mitomycin C.

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