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Autofocus evaluation for brightfield microscopy pathology.

Authors
  • Redondo, Rafael
  • Bueno, Gloria
  • Valdiviezo, Juan Carlos
  • Nava, Rodrigo
  • Cristóbal, Gabriel
  • Déniz, Oscar
  • García-Rojo, Marcial
  • Salido, Jesus
  • Fernández, Maria del Milagro
  • Vidal, Juan
  • Escalante-Ramírez, Boris
Type
Published Article
Journal
Journal of Biomedical Optics
Publisher
SPIE - International Society for Optical Engineering
Publication Date
Mar 01, 2012
Volume
17
Issue
3
Pages
36008–36008
Identifiers
DOI: 10.1117/1.JBO.17.3.036008
PMID: 22502566
Source
Medline
License
Unknown

Abstract

An essential and indispensable component of automated microscopy framework is the automatic focusing system, which determines the in-focus position of a given field of view by searching the maximum value of a focusing function over a range of z-axis positions. The focus function and its computation time are crucial to the accuracy and efficiency of the system. Sixteen focusing algorithms were analyzed for histological and histopathological images. In terms of accuracy, results have shown an overall high performance by most of the methods. However, we included in the evaluation study other criteria such as computational cost and focusing curve shape which are crucial for real-time applications and were used to highlight the best practices.

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