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Autofluorescence as a Signal to Sort Developing Glandular Trichomes by Flow Cytometry.

Authors
  • Bergau, Nick1
  • Navarette Santos, Alexander2
  • Henning, Anja1
  • Balcke, Gerd U1
  • Tissier, Alain1
  • 1 Department of Cell and Metabolic Biology, Leibniz-Institute of Plant Biochemistry Halle, Germany. , (Germany)
  • 2 Center for Basic Medical Research, Martin-Luther University of Halle-Wittenberg Halle, Germany. , (Germany)
Type
Published Article
Journal
Frontiers in Plant Science
Publisher
Frontiers Media SA
Publication Date
Jan 01, 2016
Volume
7
Pages
949–949
Identifiers
DOI: 10.3389/fpls.2016.00949
PMID: 27446176
Source
Medline
Keywords
License
Unknown

Abstract

The industrial relevance of a number of metabolites produced in plant glandular trichomes (GTs) has spurred research on these specialized organs for a number of years. Most of the research, however, has focused on the elucidation of secondary metabolite pathways and comparatively little has been undertaken on the development and differentiation of GTs. One way to gain insight into these developmental processes is to generate stage-specific transcriptome and metabolome data. The difficulty for this resides in the isolation of early stages of development of the GTs. Here we describe a method for the separation and isolation of intact young and mature type VI trichomes from the wild tomato species Solanum habrochaites. The final and key step of the method uses cell sorting based on distinct autofluorescence signals of the young and mature trichomes. We demonstrate that sorting by flow cytometry allows recovering pure fractions of young and mature trichomes. Furthermore, we show that the sorted trichomes can be used for transcript and metabolite analyses. Because many plant tissues or cells have distinct autofluorescence components, the principles of this method can be generally applicable for the isolation of specific cell types without prior labeling.

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