Autoantibody profiles in the sera of patients with Q fever: characterization of antigens by immunofluorescence, immunoblot and sequence analysis

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Autoantibody profiles in the sera of patients with Q fever: characterization of antigens by immunofluorescence, immunoblot and sequence analysis

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BioMed Central
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PMC
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  • Biology
  • Medicine
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Abstract

1740-2557-2-10.fm ral ss BioMed CentJournal of Autoimmune Diseases Open AcceResearch Autoantibody profiles in the sera of patients with Q fever: characterization of antigens by immunofluorescence, immunoblot and sequence analysis MT Camacho1, I Outschoorn2, A Tellez1 and J Sequí*3 Address: 1Departamento de Orientación Diagnóstica. Centro Nacional de Microbiologia. Instituto de Salud Carlos III. Ctra. Majadahonda - Pozuelo Km 12,5. 28080-Madrid. Spain, 2Departamento de Respuesta Inmune. Centro Nacional de Microbiologia. Instituto de Salud Carlos III. Ctra. Majadahonda -Pozuelo Km 12,5. 28080-Madrid. Spain and 3Servicio de Inmunología. Hospital Carlos III. Imsalud. c/ Sinesio Delgado n° 10. 28029-Madrid. Spain Email: MT Camacho - [email protected]; I Outschoorn - [email protected]; A Tellez - [email protected]; J Sequí* - [email protected] * Corresponding author AutoantibodiesC. burnetiiQ fever. Abstract Recent reports have shown that some of the immunological aspects of Q fever, a rickettsiosis caused by Coxiella burnetii, could be related to self-antigen responses. The aim of this study was to determine the specificity of the autoantibody response of patients with acute and chronic Coxiella infections. Smooth muscle and cardiac muscle-specific autoantibodies were observed in significant percentages in acutely or chronically affected Q fever patients when compared to healthy volunteers. Moreover, the incidence of cardiac muscle-specific autoantibody was significantly higher among chronically ill patients compared to acutely ill patients. Moreover, a band of 50 kD of a HeLa extract was detected in most of the sera of individuals with chronic infections and previous sequence analysis suggests that this antigen presents a high degree of homology with the human actin elongation factor 1 alpha. Further research would be necessary to confirm if antibodies to human cytoskeletal proteins could be of clinical importance in chronically infected Q fever patients

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