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AuthentiCT: a model of ancient DNA damage to estimate the proportion of present-day DNA contamination

Authors
  • Peyrégne, Stéphane1
  • Peter, Benjamin M.1
  • 1 Max Planck Institute for Evolutionary Anthropology, Leipzig, 04103, Germany , Leipzig (Germany)
Type
Published Article
Publication Date
Sep 15, 2020
Volume
21
Issue
1
Identifiers
DOI: 10.1186/s13059-020-02123-y
Source
Springer Nature
Keywords
License
Green

Abstract

Contamination from present-day DNA is a fundamental issue when studying ancient DNA from historical or archaeological material, and quantifying the amount of contamination is essential for downstream analyses. We present AuthentiCT, a command-line tool to estimate the proportion of present-day DNA contamination in ancient DNA datasets generated from single-stranded DNA libraries. The prediction is based solely on the patterns of post-mortem damage observed on ancient DNA sequences. The method has the power to quantify contamination from as few as 10,000 mapped sequences, making it particularly useful for analysing specimens that are poorly preserved or for which little data is available.

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