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ATP-dependent looping of DNA by ISWI.

Authors
  • Lia, Giuseppe1
  • Indrieri, Marco
  • Owen-Hughes, Tom
  • Finzi, Laura
  • Podesta, Alessandro
  • Milani, Paolo
  • Dunlap, David
  • 1 Harvard University, Chemistry & Chemical Biology Dept., Cambridge, MA, USA.
Type
Published Article
Journal
Journal of Biophotonics
Publisher
Wiley (John Wiley & Sons)
Publication Date
September 2008
Volume
1
Issue
4
Pages
280–286
Identifiers
DOI: 10.1002/jbio.200810027
PMID: 19343651
Source
Medline
License
Unknown

Abstract

Snf2 related chromatin remodelling enzymes possess an ATPase subunit similar to that of the SF-II helicases which hydrolyzes ATP to track along DNA. Translocation and any resulting torque in the DNA could drive chromatin remodeling. To determine whether the ISWI protein can translocate and generate torque, tethered particle motion experiments and atomic force microscopy have been performed using recombinant ISWI expressed in E. coli. In the absence of ATP, ISWI bound to and wrapped DNA thereby shortening the overall contour length measured in atomic force micrographs. Although naked DNA only weakly stimulates ATP hydrolysis by ISWI, both atomic force microscopy and tethered particle motion data indicate that the protein generated loops in the presence of ATP. The duration of the looped state of the DNA measured using tethered particle motion was ATP-dependent. Finally, ISWI relaxed positively supercoiled plasmids visualized by atomic force microscopy. While other chromatin remodeling ATPases catalyze either DNA wrapping or looping, both are catalyzed by ISWI.

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