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Associations between clinical canine leishmaniosis and multiple vector-borne co-infections: a case-control serological study

Authors
  • Attipa, Charalampos1, 2, 3, 4
  • Solano-Gallego, Laia5
  • Leutenegger, Christian M.6
  • Papasouliotis, Kostas1, 7
  • Soutter, Francesca2
  • Balzer, Jörg8
  • Carver, Scott9
  • Buch, Jesse S.10
  • Tasker, Séverine1, 11, 12
  • 1 Bristol Veterinary School and Langford Vets, University of Bristol, Molecular Diagnostic Unit, Diagnostic Laboratories, Langford, UK , Langford (United Kingdom)
  • 2 University of London, Department of Pathobiology and Population Sciences, The Royal Veterinary College, Hatfield, Hertfordshire, UK , Hatfield (United Kingdom)
  • 3 Cyvets Veterinary Center, Paphos, Cyprus , Paphos (Cyprus)
  • 4 Institute of Infection and Global Health, University of Liverpool, Department of Clinical Infection, Microbiology and Immunology, Liverpool, UK , Liverpool (United Kingdom)
  • 5 Universitat Autònoma de Barcelona, Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Barcelona, Spain , Barcelona (Spain)
  • 6 IDEXX Laboratories, Inc., West Sacramento, CA, USA , West Sacramento (United States)
  • 7 Present Address: IDEXX Laboratories Ltd., Wetherby, UK , Wetherby (United Kingdom)
  • 8 IDEXX GmbH, Ludwigsburg, Germany , Ludwigsburg (Germany)
  • 9 University of Tasmania, Department of Biological Sciences, Hobart, TAS, Australia , Hobart (Australia)
  • 10 IDEXX Laboratories, Inc., Westbrook, ME, USA , Westbrook (United States)
  • 11 University of Bristol, Bristol Veterinary School, Langford, UK , Langford (United Kingdom)
  • 12 The Linnaeus Group, Shirley, UK , Shirley (United Kingdom)
Type
Published Article
Journal
BMC Veterinary Research
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Sep 18, 2019
Volume
15
Issue
1
Identifiers
DOI: 10.1186/s12917-019-2083-6
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundDogs that have clinical leishmaniosis (ClinL), caused by the parasite Leishmania infantum, are commonly co-infected with other pathogens, especially vector-borne pathogens (VBP). A recent PCR-based study found that ClinL dogs are more likely to be additionally infected with the rickettsial bacteria Ehrlichia canis. Further information on co-infections in ClinL cases with VBP, as assessed by serology, is required. The research described in this report determined if dogs with ClinL are at higher risk of exposure to VBP than healthy control dogs using a case-control serology study.ResultsOf the 47 dogs with ClinL, anti-E. canis/ Ehrlichia ewingii antibodies were detected in 17 (36.2%), anti-Anaplasma phagocytophilum/Anaplasma platys antibodies in 5 (10.6%) and antigen for Dirofilaria immitis in 2 (4.3%). Of the 87 control dogs, anti-E. canis/E. ewingii antibodies were detected in 14 (16.1%) and anti-A. phagocytophilum/A. platys antibodies in 2 (2.3%). No anti-Borrelia burgdorferi antibody tests were positive. No statistical differences between the ClinL dogs and control dogs regarding lifestyle or use of ectoparasitic prevention, were identified. The ClinL was significantly associated with anti-E. canis/E. ewingii antibodies (odds ratio = 2.9, 95% confidence interval: 1.3–6.7, P = 0.010) compared to controls by both multivariable logistic regression and structural equation modelling.ConclusionsIt was demonstrated that an increased risk for E. canis/E. ewingii seropositivity is present in dogs with ClinL compared to clinically healthy control dogs, despite similar ectoparasitic prevention use and lifestyle. Based on these findings it is suggested that dogs with ClinL should not only be tested for E. canis co-infection using PCR but also serologically for E. canis/E. ewingii.

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