Affordable Access

Association of ABCA2 expression with determinants of Alzheimer s disease.

Authors
  • Zj, Chen
  • B, Vulevic
  • Ke, Ile
  • Athena Soulika
  • Jr, Davis W
  • Pb, Reiner
  • Bp, Connop
  • P, Nathwani
  • Jq, Trojanowski
  • Kd, Tew
Type
Published Article
Journal
The FASEB Journal
Publisher
Federation of American Society for Experimental Biology
Volume
18
Issue
10
Pages
1129–1129
Source
Soulika Lab - UC Davis dermatology-ucdavis
License
Unknown

Abstract

With the use of a novel method for detecting differential gene expression, alterations in functional gene clusters related to transport or oxidative stress response and beta-amyloid (Abeta) peptide metabolism were identified in a HEK293 cell line engineered to overexpress the human ATP binding cassette transporter ABCA2. These included fatty acid binding protein, phospholipid binding protein, phospholipid synthesis protein, transporter cofactors, seladin-1, Abeta precursor protein (APP), vimentin, and low-density lipoprotein receptor-related protein. ABCA2 was highly expressed in neuroblastoma cells and colocalized with Abeta and APP. Additionally, increased APP protein levels were detected within ABCA2/APP double-transfected cells, and increased Abeta was detected in the media of ABCA2-transfected cells relative to controls. The transporter was abundant in the temporal and frontal regions of both normal and Alzheimer s disease (AD) brain but was detected at lower concentrations in the parietal, occipital, and cerebellar regions. The ABCA2 transfected cell line expressed resistance to a free radical initiator, confirming involvement in protection against reactive oxygen species and suggesting a further possible link to AD.

Report this publication

Statistics

Seen <100 times