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Assessment of Statin Interactions With the Human NTCP Transporter Using a Novel Fluorescence Assay.

Authors
  • Wang, Ying1
  • Wilkerson, Matthew2
  • Li, Jibin1
  • Zhang, Wei1
  • Owens, Albert1
  • Wright, Stephen2
  • Hidalgo, Ismael1
  • 1 376544Absorption Systems LP, Exton, PA, USA.
  • 2 4140Carson-Newman University, Jefferson City, TN, USA.
Type
Published Article
Journal
International journal of toxicology
Publication Date
Jan 01, 2020
Volume
39
Issue
6
Pages
518–529
Identifiers
DOI: 10.1177/1091581820953066
PMID: 33078647
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Sodium taurocholate cotransporting polypeptide (NTCP), which is highly expressed in the sinusoidal membrane of hepatocytes, maintains bile acid homeostasis and participates in the hepatic disposition of a variety of endogenous substances as well as xenobiotics. Manifested by the involvement of organic anion-transporting polypeptides 1B1 and 1B3 (OATP1B1 and OATP1B3) in the hepatic uptake of statin drugs, sinusoidal membrane transporters play an important role in the pharmacokinetics and pharmacodynamics of these agents. It has been speculated that NTCP may function as an alternative pathway for statin hepatic uptake, complementary to OATP1B1 and OATP1B3. In the current study, we produced stable NTCP-expressing human embryonic kidney 293 (HEK293) cells and developed a fluorescence-based assay using flow cytometry for measuring NTCP transport with chenodeoxycholyl-(Nε-7-nitrobenz-2-oxa-1,3-diazole)-lysine (CDCA-NBD) as the substrate. NTCP-mediated CDCA-NBD transport was time-dependent and exhibited typical Michaelis-Menten kinetics, with a K m of 6.12 µM. Compounds known to interact with NTCP, including chenodeoxycholic acid and taurocholic acid, displayed concentration-dependent inhibition of NTCP-mediated CDCA-NBD transport. We report here a systematic evaluation of the interaction between statins and the NTCP transporter. Utilizing this system, several statins were either found to inhibit NTCP-dependent transport or act as substrates. We find a good correlation between the reported lipophilicity of statins and their ability to inhibit NTCP. The objective was to develop a higher-throughput system to evaluate potential inhibitors such as the statins. The in vitro assays using CDCA-NBD as fluorescent substrate are convenient, rapid, and have utility in screening drug candidates for potential drug-NTCP interactions.

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