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Assessment of the role in protection and pathogenesis of the Chlamydia muridarum V-type ATP synthase subunit A (AtpA) (TC0582)

Authors
  • Cheng, Chunmei
  • Jain, Pooja
  • Pal, Sukumar
  • Tifrea, Delia
  • Sun, Guifeng
  • Teng, Andy A.
  • Liang, Xiaowu
  • Felgner, Philip L.
  • de la Maza, Luis M.1, 2, 3, 4, 5, 3
  • 1 Department of Pathology and Laboratory Medicine
  • 2 Medical Sciences I
  • 3 University of California, Irvine
  • 4 ImmPORT Therapeutics, Inc./Antigen Discovery Inc.
  • 5 Department of Medicine
Type
Published Article
Journal
Microbes and Infection
Publisher
Elsevier
Publication Date
Jan 01, 2013
Accepted Date
Oct 14, 2013
Volume
16
Issue
2
Pages
123–133
Identifiers
DOI: 10.1016/j.micinf.2013.10.012
Source
Elsevier
Keywords
License
Unknown

Abstract

A novel Chlamydia muridarum antigen (TC0582) was used to vaccinate BALB/c mice. Mice were also immunized with other components of the ATP synthase complex (TC0580, TC0581, and TC0584), or with the major outer membrane protein (MOMP). TC0582 was also formulated in combination with TC0580, TC0581 or MOMP. TC0582 alone, or in combination with the other antigens, elicited strong Chlamydia-specific humoral and cellular immune responses. Vaccinated animals were challenged intranasally and the course of the infection was followed for 10 days. Based on percentage change in body weight, lung weight, and number of Chlamydia inclusion forming units recovered from the lungs, mice immunized with TC0582, TC0581 or MOMP, as single antigens, showed significant protection. Mice immunized with combinations of two antigens were also protected but the level of protection was not additive. TC0582 has sequence homology with the eukaryotic ATP synthase subunit A (AtpA). Therefore, to determine if immunization with TC0582, or with Chlamydia, elicited antibodies that cross-reacted with the mouse AtpA, the two proteins were printed on a microarray. Sera from mice immunized with TC0582 and/or live Chlamydia, strongly reacted with TC0582 but did not recognize the mouse AtpA. In conclusion, TC0582 may be considered as a Chlamydia vaccine candidate.

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