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Assay optimisation and age-related baseline variation in biochemical markers in Lesser Black-backed gulls.

Authors
  • Santos, Cátia S A1
  • Loureiro, Susana2
  • Sotillo, Alejandro3
  • Müller, Wendt4
  • Stienen, Eric W M5
  • De Neve, Liesbeth6
  • Lens, Luc6
  • Monteiro, Marta S2
  • 1 Department of Biology, Terrestrial Ecology Unit, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium; Department of Biology & CESAM, Centre for Environmental and Marine Studies, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal. Electronic address: [email protected] , (Belgium)
  • 2 Department of Biology & CESAM, Centre for Environmental and Marine Studies, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal. , (Portugal)
  • 3 Department of Biology, Terrestrial Ecology Unit, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium; Department of Biology & CESAM, Centre for Environmental and Marine Studies, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal. , (Belgium)
  • 4 Department of Biology - Behavioural Ecology and Ecophysiology Group, University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, 2610 Wilrijk, Antwerp, Belgium. , (Belgium)
  • 5 Research Institute for Nature and Forest (INBO), Havenlaan 88 Bus 73, 1000 Brussels, Belgium. , (Belgium)
  • 6 Department of Biology, Terrestrial Ecology Unit, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium. , (Belgium)
Type
Published Article
Journal
Ecotoxicology and Environmental Safety
Publisher
Elsevier
Publication Date
May 15, 2019
Volume
172
Pages
246–254
Identifiers
DOI: 10.1016/j.ecoenv.2019.01.084
PMID: 30711859
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Free-ranging animals are often used as bioindicators of both short- and long-term changes in ecosystem health, mainly to detect the presence and effects of contaminants. Birds, and gulls in particular, have been used as bioindicators over a broad range of marine and terrestrial ecosystems. In this study, we standardise the conditions for the use of a suite of biochemical markers in non-destructive matrices of Lesser Black-backed Gull (Larus fuscus) to facilitate future biomonitoring of marine and terrestrial contaminants. We characterized cholinesterase (ChE) in plasma and optimized assay conditions for ChE activity as a marker of neurotoxic damage. Moreover, we quantified variation in activity of ChE, lactate dehydrogenase (LDH), glutathione-S-transferase (GST) and catalase (CAT) as well as variation ranges of lipid peroxidation (LPO), in free-ranging adults and captive chicks. The main ChE form present in plasma of both adults and chicks was butyrylcholinesterase (BChE) followed by acetylcholinesterase (AChE), whose relative proportion in plasma tended to decrease with increased chick age. LPO levels and GST activity in blood cells (BCs) decreased significantly with increasing chick age, while BChE and LDH activity in plasma were not age-dependent. CAT in BCs tended to decline non-significantly in older chicks. Results of this study underscore the importance of standardising assay conditions and assessing intrinsic baseline variation in biochemical markers, before biochemical quantification. Data presented here provide a foundation for future use of BChE and LDH activity in plasma, as well as oxidative stress markers (LPO, CAT and GST) in BCs, to monitor environmental stress effects in Lesser Black-backed gulls. Copyright © 2019. Published by Elsevier Inc.

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