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Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides.

Authors
  • Abukar, Tasnim1
  • Buenbrazo, Nakita1
  • Janesch, Bettina1
  • Kell, Laura1
  • Wakarchuk, Warren2
  • 1 Department of Chemistry and Biology, Ryerson University, Toronto, ON, Canada. , (Canada)
  • 2 Department of Chemistry and Biology, Ryerson University, Toronto, ON, Canada. [email protected] , (Canada)
Type
Published Article
Journal
Methods in molecular biology (Clifton, N.J.)
Publication Date
Jan 01, 2019
Volume
1954
Pages
215–235
Identifiers
DOI: 10.1007/978-1-4939-9154-9_17
PMID: 30864135
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Glycans play many important roles in bacterial biology and the complexity of the glycan structures requires biochemical assays in place to help characterize the biosynthetic pathways. Our focus has been on the use of enzymes from pathogens which make molecular mimics of host glycans. We have been examining glycosyltransferases that make strategic linkages in biologically active glycans which can be also exploited for potential therapeutic glycoconjugate synthesis. This chapter will provide details on assays for a variety of bacterial glycosyltransferases that we and others have used for the characterization of pathogen glycoconjugate biosynthetic pathways, and for the in vitro synthesis of human-like glycans produced by bacterial pathogens. The methods presented here should enable other assays to be developed for new pathway characterization.

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