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Ascorbic and dehydroascorbic acid measurement in human serum and plasma.

Authors
  • Margolis, S A1
  • Ziegler, R G
  • Helzlsouer, K J
  • 1 National Institute of Standards & Technology, Gaithersburg, MD 20899.
Type
Published Article
Journal
The American journal of clinical nutrition
Publication Date
Dec 01, 1991
Volume
54
Issue
6 Suppl
Identifiers
DOI: 10.1093/ajcn/54.6.1315s
PMID: 1962589
Source
Medline
License
Unknown

Abstract

Plasma supplemented with ascorbic acid was prepared; the stability of these samples was characterized and the accuracy of the supplementation was established. Studies on the accuracy, precision, and sources of methodological bias in the measurement of ascorbic acid were summarized. Measurements of the ratio of ascorbic acid to dehydroascorbic acid in clinical samples was evaluated and was shown to be relatively constant in plasma taken from blood stored at 12 degrees C for 6 h. These results imply that whole blood has the capacity to maintain a constant ascorbic-dehydroascorbic acid ratio and suggest that this ratio may be of physiological significance.

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