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Artyfechinostomum malayanum: Metacercariae Encysted in Pila sp. Snails Purchased from Phnom Penh, Cambodia.

Authors
  • Sohn, Woon-Mok1
  • Yong, Tai-Soon2
  • Eom, Keeseon S3
  • Sinuon, Muth4
  • Jeoung, Hoo-Gn5
  • Chai, Jong-Yil5, 6
  • 1 Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 52727, Korea. , (North Korea)
  • 2 Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea. , (North Korea)
  • 3 Department of Parasitology, Medical Research Institute and Parasite Resource Bank, School of Medicine, Chungbuk National University, Cheongju 28644, Korea. , (North Korea)
  • 4 Center for National Malaria Control, Phnom Penh, Cambodia. , (Cambodia)
  • 5 Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07653, Korea. , (North Korea)
  • 6 Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Korea. , (North Korea)
Type
Published Article
Journal
The Korean journal of parasitology
Publication Date
Jun 01, 2017
Volume
55
Issue
3
Pages
341–345
Identifiers
DOI: 10.3347/kjp.2017.55.3.341
PMID: 28719961
Source
Medline
Keywords
License
Unknown

Abstract

The metacercariae of Artyfechinostomum malayanum (Leiper, 1911) Mendheim, 1943 were discovered in Pila sp. snails purchased from a market in Phnom Penh, Cambodia. They were isolated from the snails using the artificial digestion technique and were orally fed to 2 hamsters, 1 rat, and 2 mice to obtain the adult flukes. The metacercariae were round, 145-165 μm in diameter, having a cyst wall of 6-10 μm in thickness, a head collar and collar spines, and characteristic features of excretory granules. Adult flukes were recovered in the small intestines of the animals at days 14 and 32 post infection and were morphologically observed using a light microscope and a scanning electron microscope. They were plump or elongated, ventrally curved, 6.0-8.1×1.6-2.0 mm in size, and characterized by the head collar bearing 43 collar spines, including 5 end group ones on each side, a long cirrus sac extending beyond the posterior margin of the ventral sucker, a submedian ovary, and 2 deeply lobed testes. Eggs in uteri were operculate, ovoid to ellipsoid, and 120-135×68-75 μm in size. In scanning electron microscopy, the head collar was prominent with collar spines looking like horns. Scale-like tegumental spines were densely distributed on the ventral surface between the head collar and ventral sucker. Sensory papillae were distributed mainly on the tegument around suckers. By this study, it has been first confirmed that the life cycle of A. malayanum exists in Cambodia.

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