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ARFGAP1 is dynamically associated with lipid droplets in hepatocytes.

Authors
  • Gannon, Joan1
  • Fernandez-Rodriguez, Julia2
  • Alamri, Hussam1
  • Feng, Shi Bo1
  • Kalantari, Fariba1
  • Negi, Sarita1
  • Wong, Amy H Y1
  • Mazur, Alexander1
  • Asp, Lennart3
  • Fazel, Ali1
  • Salman, Ayat1
  • Lazaris, Anthoula1
  • Metrakos, Peter1
  • Bergeron, John J M1
  • Nilsson, Tommy1
  • 1 The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada. , (Canada)
  • 2 The Centre for Cellular Imaging at the University of Gothenburg, Gothenburg, Sweden. , (Sweden)
  • 3 The Department of Medical and Clinical Genetics, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden. , (Sweden)
Type
Published Article
Journal
PLoS ONE
Publisher
Public Library of Science
Publication Date
Jan 01, 2014
Volume
9
Issue
11
Identifiers
DOI: 10.1371/journal.pone.0111309
PMID: 25397679
Source
Medline
License
Unknown

Abstract

The ARF GTPase Activating Protein 1 (ARFGAP1) associates mainly with the cytosolic side of Golgi cisternal membranes where it participates in the formation of both COPI and clathrin-coated vesicles. In this study, we show that ARFGAP1 associates transiently with lipid droplets upon addition of oleate in cultured cells. Also, that addition of cyclic AMP shifts ARFGAP1 from lipid droplets to the Golgi apparatus and that overexpression and knockdown of ARFGAP1 affect lipid droplet formation. Examination of human liver tissue reveals that ARFGAP1 is found associated with lipid droplets at steady state in some but not all hepatocytes.

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