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The Arf family G protein Arl1 is required for secretory granule biogenesis in Drosophila.

Authors
  • Torres, Isabel L
  • Rosa-Ferreira, Cláudia
  • Munro, Sean
Type
Published Article
Journal
Journal of Cell Science
Publisher
The Company of Biologists
Publication Date
May 15, 2014
Volume
127
Issue
Pt 10
Pages
2151–2160
Identifiers
DOI: 10.1242/jcs.122028
PMID: 24610947
Source
Medline
Keywords
License
Unknown

Abstract

The small G protein Arf like 1 (Arl1) is found at the Golgi complex, and its GTP-bound form recruits several effectors to the Golgi including GRIP-domain-containing coiled-coil proteins, and the Arf1 exchange factors Big1 and Big2. To investigate the role of Arl1, we have characterised a loss-of-function mutant of the Drosophila Arl1 orthologue. The gene is essential, and examination of clones of cells lacking Arl1 shows that it is required for recruitment of three of the four GRIP domain golgins to the Golgi, with Drosophila GCC185 being less dependent on Arl1. At a functional level, Arl1 is essential for formation of secretory granules in the larval salivary gland. When Arl1 is missing, Golgi are still present but there is a dispersal of adaptor protein 1 (AP-1), a clathrin adaptor that requires Arf1 for its membrane recruitment and which is known to be required for secretory granule biogenesis. Arl1 does not appear to be required for AP-1 recruitment in all tissues, suggesting that it is crucially required to enhance Arf1 activation at the trans-Golgi in particular tissues.

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