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Arabidopsis CYP98A3 Mediating Aromatic 3-Hydroxylation. Developmental Regulation of the Gene, and Expression in Yeast1

Authors
  • Ramesh B. Nair
  • Qun Xia
  • Cyril J. Kartha
  • Eugen Kurylo
  • Rozina N. Hirji
  • Raju Datla
  • Gopalan Selvaraj
Publisher
American Society of Plant Physiologists
Publication Date
Sep 01, 2002
Source
PMC
Keywords
Disciplines
  • Biology
  • Chemistry
License
Unknown

Abstract

Arabidopsis CYP98A3 Mediating Aromatic 3-Hydroxylation. Developmental Regulation of the Gene, and Expression in Yeast by Ramesh B. Nair, Qun Xia, Cyril J. Kartha, Eugen Kurylo, Rozina N. Hirji, Raju Datla, and Gopalan Selvaraj Plantphysiol Volume 130(1):210-220 September 1, 2002 ©2002 by American Society of Plant Biologists Biochemical transformations of the ring and side chain in cinnamic acid. Nair R et al. Plantphysiol 2002;130:210-220 ©2002 by American Society of Plant Biologists Expression patterns of some Arabidopsis CYP genes. Nair R et al. Plantphysiol 2002;130:210-220 ©2002 by American Society of Plant Biologists Hydroxylation of p-coumaric acid by yeast WAT21 (pRAM51) cells that produce Arabidopsis CYP98A3. Nair R et al. Plantphysiol 2002;130:210-220 ©2002 by American Society of Plant Biologists CYP98A3-catalyzed production of caffeic acid in yeast WAT 21 cells. Nair R et al. Plantphysiol 2002;130:210-220 ©2002 by American Society of Plant Biologists Developmental regulation of CYP98A3 andC4H. Nair R et al. Plantphysiol 2002;130:210-220 ©2002 by American Society of Plant Biologists CYP98A3 localization, and lignification. Nair R et al. Plantphysiol 2002;130:210-220 ©2002 by American Society of Plant Biologists Biochemical transformations of the ring and side chain in cinnamic acid. It is the first phenolic acid in the general phenylpropanoid metabolism arising from deamination ofl-Phe by PAL. 4-Hydroxylation of cinnamic acid by C4H generates p-coumaric acid, but further ring transformations can potentially occur when the γ-carbon is an acid, aldehyde, or ester. In the current depiction of the pathways, 3-hydroxylation occurs on p-coumaric acid or p-coumaroyl CoA, and the later 5-hydroxylation by ferulic acid 5-hydroxylase (F5H) occurs when the γ-carbon is in aldehyde or alcohol form. A fully methoxylated acid, i.e. 4-hydroxy,3,5-dimethoxycinnamic acid (sinapic acid) is an intermediate in sinapine and other sinapate ester synthesis. The gene encoding 3-hydroxylatio

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