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ARABIDOPSIS CRINKLY4 Function, Internalization, and Turnover Are Dependent on the Extracellular Crinkly Repeat DomainW⃞

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Publisher
American Society of Plant Biologists
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PMC
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  • Biology
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Abstract

ARABIDOPSIS CRINKLY4 Function, Internalization, and Turnover Are Dependent on the Extracellular Crinkly Repeat Domain by Miriam L. Gifford, Fiona C. Robertson, Dinesh C. Soares, and Gwyneth C. Ingram Plantcell Volume 17(4):1154-1166 April 1, 2005 ©2005 by American Society of Plant Biologists Behavior of ACR4:GFP in Root Cells.(A) to (I) Root cells of plants carrying the complementing ACR4:GFP fusion protein and treated with FM4-64. Gifford M et al. Plantcell 2005;17:1154-1166 ©2005 by American Society of Plant Biologists ACR4 Derivatives Used for Complementation Analysis. Gifford M et al. Plantcell 2005;17:1154-1166 ©2005 by American Society of Plant Biologists The ACR4 TNFR-Like Domain and Crinkly Repeats Are Targeted to the Extracellular Space.Ovules expressing ACR4:GFP ([A] and [B]) and ΔTM/KIN/C-TER:GFP ([C] and [D]). Gifford M et al. Plantcell 2005;17:1154-1166 ©2005 by American Society of Plant Biologists Three-Dimensional Model of the ACR4 Crinkly Repeat Domain.PyMol (http://www.pymol.org) illustration of the three-dimensional model structure of the crinkly repeat domain of ACR4, with secondary structure assigned by the standard settings within PyMol. Gifford M et al. Plantcell 2005;17:1154-1166 ©2005 by American Society of Plant Biologists Cellular Behavior of Nonfunctional ACR4 Variants.Visualization of GFP ([A] and [D]), FM4-64 ([B] and [E]), and merged images ([C] and [F]) of root cells from plants carrying the ΔREPEAT:GFP construct. Gifford M et al. Plantcell 2005;17:1154-1166 ©2005 by American Society of Plant Biologists Protein Gel Blot Analysis of GFP Protein Fusions.Protein gel blot analysis of proteins isolated from plants carrying GFP-tagged ACR4 derivatives using an anti-GFP polyclonal antibody. Gifford M et al. Plantcell 2005;17:1154-1166 ©2005 by American Society of Plant Biologists Behavior of ACR4:GFP in Root Cells.(A) to (I) Root cells of plants carrying the complementing ACR4:GFP fusion protein and treated with FM4-64. Cells were simultaneously obser

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