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Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation

Authors
  • Suhua Feng
  • Yunping Shen
  • James A. Sullivan
  • Vicente Rubio
  • Yue Xiong
  • Tai-ping Sun
  • Xing Wang Deng
Publisher
American Society of Plant Biologists
Publication Date
Jul 01, 2004
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

STANDARD OPERATING PROCEDURE ��http://proteomics.cancer.gov STANDARD OPERATING PROCEDURE Title: Antibody Production at Precision Antibody, Inc. SOP#: M-124 Version #: 1 Author: Precision Antibody, Inc. Date Approved: Date Modified: PURPOSE This procedure is to describe the methods used by Preicsion Laboratory, Incto produce and grow up monoclonal antibodies for the Clinical Proteomic Technologies for Cancer (CPTC) program. SCOPE This document describes the procedure for production and growth of monoclonal antibodies to specific cancer related target proteins. RESPONSIBILITIES It is the responsibility of the person(s) preparing these reagents to be familiar with lab safety procedures and to have basic laboratory skills. Equipment Reagents Antigen for desired cancer related protein Procedure Immunization Strategy SOP in Preparation Cellular Fusion SOP in Preparation Limited Dilution Cloning SOP in preparation Freezing Suspension Cell Lines SOP in Preparation Direct ELISA SOP in Preparation . ReferenceD Documents SOP in preparation M-124.1 Page � PAGE �1� of � NUMPAGES �2�

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