Affordable Access

deepdyve-link
Publisher Website

APTM, a Thiophene Heterocyclic Compound, Inhibits Human Colon Cancer HCT116 Cell Proliferation Through p53-Dependent Induction of Apoptosis.

Authors
  • Liao, Xiaolin1, 2
  • Huang, Jiajun1
  • Lin, Wanjun1
  • Long, Ze1
  • Xie, Ying1
  • Ma, Wenzhe1
  • 1 1 State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology , Macau, China . , (China)
  • 2 2 Department of Pharmacy, People's Hospital of Yicheng , Hubei, China . , (China)
Type
Published Article
Journal
DNA and Cell Biology
Publisher
Mary Ann Liebert
Publication Date
Dec 07, 2017
Identifiers
DOI: 10.1089/dna.2017.3962
PMID: 29215922
Source
Medline
Keywords
License
Unknown

Abstract

To evaluate the in vitro anticancer activity and to investigate the mechanism of action of a thiophene heterocyclic compound, [3-Amino-5-[(2,6-dimethylphenyl)amino]-4-(phenylsulfonyl)-2-thienyl](4-fluorophenyl)methanone (APTM) against human colon cancer HCT116 cells. Sulforhodamine B assay and colony formation assay for cell proliferation assay; propidium iodide (PI) staining for cell cycle profile analysis; Hoechst staining; annexin V-FITC/PI double staining and Western blotting for apoptosis assay. APTM inhibits the growth of HCT116 cells dose and time dependently. The growth inhibitory effect of APTM on HCT116 cells was associated with induction of apoptosis but not cell cycle arrest. Also, the isogenic cell depletion of p53 was resistant to APTM-induced apoptosis and thus grows relatively better than the wild-type cells. The anticancer effect of APTM resulted from p53-dependent induction of apoptosis. Also, APTM is a promising lead compound for the treatment of human colon cancer.

Report this publication

Statistics

Seen <100 times