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Applying proteomic technology to clinical virology.

Authors
  • Mancone, C1
  • Ciccosanti, F2
  • Montaldo, C3
  • Perdomo, A B2
  • Piacentini, M4
  • Alonzi, T2
  • Fimia, G M2
  • Tripodi, M5
  • 1 'Lazzaro Spallanzani' National Institute for Infectious Diseases I.R.C.C.S.; Department of Cellular Biotechnologies and Haematology, Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University of Rome. Electronic address: [email protected]
  • 2 'Lazzaro Spallanzani' National Institute for Infectious Diseases I.R.C.C.S.
  • 3 'Lazzaro Spallanzani' National Institute for Infectious Diseases I.R.C.C.S.; Department of Cellular Biotechnologies and Haematology, Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University of Rome.
  • 4 'Lazzaro Spallanzani' National Institute for Infectious Diseases I.R.C.C.S.; Department of Biology, University of Rome 'Tor Vergata', Rome, Italy. , (Italy)
  • 5 'Lazzaro Spallanzani' National Institute for Infectious Diseases I.R.C.C.S.; 'Lazzaro Spallanzani' National Institute for Infectious Diseases I.R.C.C.S.
Type
Published Article
Journal
Clinical Microbiology and Infection
Publisher
Elsevier
Publication Date
Jan 01, 2013
Volume
19
Issue
1
Pages
23–28
Identifiers
DOI: 10.1111/1469-0691.12029
PMID: 23034105
Source
Medline
License
Unknown

Abstract

Developing antiviral drugs, vaccines and diagnostic markers is still the most ambitious challenge in clinical virology. In the past few decades, data from high-throughput technologies have allowed for the rapid development of new antiviral therapeutic strategies, thus making a profound impact on translational research. Most of the current preclinical studies in virology are aimed at evaluating the dynamic composition and localization of the protein platforms involved in various host-virus interactions. Among the different possible approaches, mass spectrometry-based proteomics is increasingly being used to define the protein composition in subcellular compartments, quantify differential protein expression among samples, characterize protein complexes, and analyse protein post-translational modifications. Here, we review the current knowledge of the most useful proteomic approaches in the study of viral persistence and pathogenicity, with a particular focus on recent advances in hepatitis C research.

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