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Applications of monoclonal antibodies to human follicle-stimulating hormone in enzyme immunoassays.

Authors
  • Chen, K W
  • Chow, S N
  • Lee, C Y
Type
Published Article
Journal
Biotechnology and applied biochemistry
Publication Date
Feb 01, 1989
Volume
11
Issue
1
Pages
83–88
Identifiers
PMID: 2495807
Source
Medline
License
Unknown

Abstract

Monoclonal antibodies against human follicle-stimulating hormone (hFSH) were generated by using an improved hybridoma technique with a semisolid medium in methylcellulose for initial cloning. The generated monoclonal antibodies were characterized with respect to their subunit and epitope specificity as well as cross-reactivity to other glycoprotein hormones. Monoclonal antibodies of high affinity and high specificity to hFSH were finally selected for applications in sandwich enzyme immunoassay. The monoclonal antibody specific to the alpha-subunit of FSH was coated on microtiter wells and served as the first antibody. The other high-affinity monoclonal antibody specific to beta-subunit of FSH was labeled with horseradish peroxidase and served as the second antibody. This immunoassay can be performed within 70 min at room temperature and has a minimum sensitivity of 2 mIU/ml for serum sample.

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