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Application of ZnO Nanorods Based Whispering Gallery Mode Resonator in Optical Immunosensors.

Authors
  • Tereshchenko, Alla1
  • Yazdi, G Reza2
  • Konup, Igor3
  • Smyntyna, Valentyn4
  • Khranovskyy, Volodymyr2
  • Yakimova, Rositsa2
  • Ramanavicius, Arunas5
  • 1 Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania. Electronic address: [email protected] , (Lithuania)
  • 2 Department of Physics, Chemistry and Biology, Linköping University, 58183 Linköping, Sweden. , (Sweden)
  • 3 Department of Microbiology, Virology and Biotechnology, Faculty of Biology, Odessa National I.I. Mechnikov University, 2, Shampanskiy Lane, 65000, Odesa, Ukraine. , (Ukraine)
  • 4 Department of Experimental Physics, Faculty of Mathematics, Physics and Information Technologies, Odesa National I.I. Mechnikov University, Pastera 42, 65023, Odesa, Ukraine. , (Ukraine)
  • 5 Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania. Electronic address: [email protected] , (Lithuania)
Type
Published Article
Journal
Colloids and surfaces. B, Biointerfaces
Publication Date
Mar 27, 2020
Volume
191
Pages
110999–110999
Identifiers
DOI: 10.1016/j.colsurfb.2020.110999
PMID: 32289650
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In this research a whispering gallery mode (WGM) resonator based on vertically oriented ZnO nanorods, which were formed on silicon surface (silicon/ZnO-NRs), has been applied in the design of optical immunosensor that was dedicated for the determination of grapevine virus A-type (GVA) proteins. Vertically oriented ZnO-NRs were grown on silicon substrates by atmospheric pressure metal organic chemical vapor deposition (APMOCVD) and the silicon/ZnO-NRs structures formed were characterized by structural and optical methods. Optical characterization demonstrates that silicon/ZnO-NRs-based structures can act as 'whispering gallery mode' (WGM) resonator where quasi-whispering gallery modes (quasi-WGMs) are generated. These quasi-WGMs were experimentally observed in the visible and infrared ranges of the photoluminescence spectra. In order to design an immuno-sensing system the anti-GVA antibodies were immobilized on the surface of silicon/ZnO-NRs and in this way silicon/ZnO-NRs/anti-GVA structure was formed. The immobilization of anti-GVA antibodies and then the interaction of silicon/ZnO-NRs/anti-GVA structure with GVA proteins (GVA-antigens) resulted in an opposite shifts of the WGMs peaks in the visible range of the photoluminescence spectra observed as a defect-related photoluminescence emission of ZnO-NRs. Here designed silicon/ZnO-NRs/anti-GVA immuno-sensing structure demonstrates the sensitivity towards GVA-antigens in the concentration range of 1-200 ng/ml. Bioanalytical applicability of the silicon/ZnO-NRs-based structures in the WGMs registration mode is discussed. Copyright © 2020 Elsevier B.V. All rights reserved.

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