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Application of recombinant antibody fragments for troponin I measurements

Authors
  • Altshuler, E. P.1
  • Vylegzhanina, A. V.2
  • Katrukha, I. A.1
  • Bereznikova, A. V.1
  • Serebryanaya, D. V.1
  • 1 Lomonosov Moscow State University, Faculty of Biology, Moscow, 119991, Russia , Moscow (Russia)
  • 2 Bialexa Ltd., Simferopolsky Ave. 8, Moscow, 117149, Russia , Moscow (Russia)
Type
Published Article
Journal
Biochemistry (Moscow)
Publisher
Pleiades Publishing
Publication Date
Dec 15, 2012
Volume
77
Issue
12
Pages
1362–1367
Identifiers
DOI: 10.1134/S0006297912120036
Source
Springer Nature
Keywords
License
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Abstract

The cardiac isoform of troponin I is a reliable biomarker of damaged cardiomyocytes that accompanies such severe cardiovascular diseases as myocardial infarction. Monoclonal antibody 19C7 recognizes troponin I in the blood-stream with high affinity and specificity. Recombinant antibodies can be used to improve detection systems based on monoclonal antibodies produced with hybridoma technology. In the present study, we compare the properties of monoclonal anti-body 19C7 and its recombinant fragments. It is shown that the recombinant antibody fragments demonstrate similar affinity values as monoclonal antibodies and can be applied for troponin I detection.

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